Microsynth standard primers software#

* = significant difference between control and BMP-2 (comparing unloaded to unloaded and loaded to loaded) # = significant difference between unloaded and loaded $ = significant difference between control unloaded and BMP-2 loaded, ( p ≤ 0. ALP) were conducted using the comparative ΔΔC T method and 18s RNA as internal control. # = significant difference between the control and the BMP-2 group (both loaded and unloaded) * = significant difference between control unloaded and control loaded $ = significantly different from the 3 days pre-culture time-point, ( p ≤ 0. The results are displayed as average ± standard deviation (n = 12). The TGF-β1 concentration was determined using a quantitative ELISA for human TGF-β1.

TGF-β1 concentration within the cell culture medium.

*Significant difference from the 3 day pre-culture time-point # = loaded sample significantly different from day 1–7 time point $ = significant difference in the unloaded vs. The BMP-2 concentration was determined using a quantitative ELISA for human BMP-2.

Microsynth standard primers software#

The primers and probes were designed and validated within our group using the primer express design software V.1.5 and were synthesized by Microsynth.īMP-2 concentration within the cell culture medium for control samples (red) and loaded samples (blue).

Self-designed forward primers, reverse primers and probes used for real-time PCR. Overview of the eight different experimental groups, which were tested in the study.